Researchers are exploring computational methods to analyze alternative polyadenylation and splicing in post-transcriptional gene regulation. Various studies are utilizing techniques such as CRISPR screens, RNA sequencing, and long read sequencing to investigate the impacts of alternative splicing on the proteome and the regulation of alternative polyadenylation. The use of these methods is providing insights into the complexities of gene regulation in different organisms, including humans, tomatoes, and pigs.