Research on the human heart has led to a greater understanding of how chromatin architecture guides the functional interpretation of disease-associated regulatory elements in cardiomyocytes. Studies have explored the epigenomics and transcriptomics of heart cells, including those in developing zebrafish and human hearts, to identify chamber-specific enhancers and gene regulatory programs. The findings have shed light on the complex regulation of cardiomyocyte development and disease, with some studies focusing on specific factors such as estrogen-related receptors and nuclear receptor elements.