Researchers are exploring the applications of multiplexed CRISPR-Cas for genome engineering, with potential uses in cereal crop improvement, plant biology, and yeast cell factory development. The technology is being developed and refined, with some studies focusing on enhancing its activity and targeting scope, while others are investigating its potential risks, including structural variations and genome integrity. The use of CRISPR-Cas for multiplex genome editing is being studied in various organisms, including yeast, and has implications for synthetic biology and metabolic pathway reconstruction.