Researchers are examining the lactating bovine mammary gland transcriptome using RNA-Sequencing, with some studies focusing on specific aspects such as milk fat globules or individual susceptibility to milk fat depression. Other studies appear to be exploring related topics, including the delivery of RNA molecules, single-cell RNA sequencing, and the benchmarking of RNA sequencing methods. The scope and goals of these studies are not entirely clear, with various approaches and applications being investigated.