Researchers are developing various methods for sorting single cells, including in-air microfluidic sorting and label-free droplet microfluidic sorting systems. These techniques aim to enhance single-cell encapsulation, morphology-guided screening, and high-throughput sorting for applications such as malignancy diagnosis and cell therapies. Different approaches, including inertial microfluidics, Raman-activated cell ejection, and acoustic, optical, and electrical methods, are being explored to improve single-cell sorting and analysis.