Research on a mucosal vaccination has shown promise in clearing Clostridioides difficile colonization. The vaccine has been tested in preclinical models, with some studies focusing on its ability to protect against infection and others examining factors that influence its effectiveness. The development of a vaccine against Clostridioides difficile is an area of ongoing research, with various approaches being explored, including multivalent mRNA-LNP vaccines and monoclonal antibody neutralization.