Researchers are using various tools and methods, including SCALPEL, to quantify transcript isoforms at the single-cell level. These approaches are being applied to study isoform diversity in different contexts, such as glioblastoma, heart failure, and endothelial-to-hematopoietic transitions. The use of long-read sequencing and computational methods is also being explored for alternative polyadenylation and splicing analysis in single-cell and spatial transcriptomics.